In Press Articles

Surgical Management of Bicornual Uterine Prolapse in a Siamese Cat: A Case Report
WK Kimani and SW Mbugua
Abstract
Abstract
Uterine prolapse is a rare condition in cats that can be managed by performing either an external hysterectomy or manual reduction followed by ovariohysterectomy. This article describes surgical management of bilateral uterine prolapse in a queen. A one year old female, pluriparous Siamese cat (Felis catus) with no past history of dystocia and weighing 2.8 kg was presented to Andys Veterinary hospital, Nairobi, Kenya with a protruding mass through the vulval. The queen had a history of recent queening and had delivered three kittens a week earlier. The owner noticed the protruding mass about 24 hours after the delivery of the last neonate. A few days later, the cat was not suckling the kittens well and was in appetent. As a sequel to this, two kittens died. After a week, the protruding mass had a pungent smell and the client presented the cat to the hospital. Complete bilateral uterine prolapse was diagnosed after a visual examination and palpation of the mass. The uterus was swollen, had necrotic areas and debris. Accordingly, a two staged ovariohysterectomy was opted for to manage the case. An internal ovariectomy was first done via a ventral midline celiotomy followed by an external hysterectomy. Post-operative management included pain medication and antibiotic therapy and the patient recovered well and was discharged after 2 days. The skin sutures were removed 10 days postoperatively. The cat recovered uneventfully with no further complications reported by the owner. In conclusion, this article shows that when the prolapsed uterus is swollen, damaged and necrotic, a two staged ovariohysterectomy should be the method of treatment and the outcome is good despite the duration of the condition.
Keywords: Felis catus, Bilateral uterine prolapse
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Flea Species Isolated from the Human and Animals: Prevalence and Ultrastructural Studies
Marwa M Attia Nagla MK Salaeh and Sohila M El-Gameel
Abstract
Abstract
Fleas are one of the insects that belong to the order Siphonaptera. They are a wingless, compressed laterally, and non-host specific insect parasitizing a wide range of hosts, so they able to transmit diseases from animals to human. This study aimed to identify the fleas in and around human and animals with their prevalence and ultrastructure study. Flea samples were collected from four governorates (Cairo; Giza; Fayoum and South Sinai); Egypt during the period from January 2017 to May 2018.One-hundred and twenty fleas were collected from five animals (donkeys, sheep, goats, dogs, and cats) surrounding humans. All the collected fleas were identified as Ctenocephalides felis felis (C. f. felis). All the animals were infested with C. f. felis with flea’s allergic dermatitis recorded in dogs, human, and donkeys, while goats and sheep showed anemic mucous membrane. The highest infestation was recorded in spring and summer. The morphological characters of 50 specimens were recorded and measured using the stereoscopic and light microscope as well as scanning electron microscope (SEM). The ultramorphological characters of C.f. felis head, thorax, and abdomen with the genital organs of male and female were fully described. The antennae have three segments with the third one had several adhesive circular disks and numerous sensory hairs in antennal grooves. The maxillary palps were well developed with four segments which supported with sensory small hairs. The first genal comb is half of the second one or nearly equal to its length. These results about identification and prevalence studies were used to update the knowledge about the flea species present in investigated localities in Egypt; to detect the appropriate control measures which could be applied in flea’s infestation.
Keywords: Ctenocephalides felis felis, Flea allergic dermatitis, Siphonaptera, Prevalence, Ultrastructure
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Effect of Marjoram Supplementation on Growth Performance and Some Immunological Indices in Broilers
Sherif Mohamed Shawky, Sahar Hassan Orabi and Ahmed Dawod
Abstract
Abstract
This study aimed to explore the effects of Marjoram (Origanum majorana L.), on growth performance, carcass characteristics, and immunity in broilers. A sixty of one-day-old Cobb broiler chicks were randomly divided into 2 groups; Chicks of group 1 were fed on basal diet considered as a control group, chicks of group 2 were fed on an experimental ration containing 2% marjoram. The results showed that the Marjoram significantly increase (P<0.05) some growth factors as body weight after 3rd, 4th and 5th weeks, weight gain at 3rd and 5th weeks, also significantly improved feed conversion ratio (FCR) at 3rd, 5th weeks in comparison with the control group. Also, Marjoram supplementation at the level of 2% caused a significant elevation (P?0.05) in gamma globulin, thus marjoram could be considered as an immunity enhancer for broilers. In conclusion, the present study proved that supplementation of the ration with Marjoram could improve growth performance and immunological state in broilers. Keywords: Marjoram, Growth performance, Immunity, Broiler
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Bio-Pesticide Control of the Brown Dog Tick (Rhipicephalus sanguineus) in Egypt by using Two Entomopathogenic Fungi (Beauveria bassiana and Metarhizium anisopliae)
Mai Mohammed Abuowarda, Mounir Abdel Haleem, Magdy Elsayed, Heba Farag and Sara Magdy
Abstract
Abstract
The current study examined the potential use of entomopathogenic fungi to control infestation of Rhipicephalus sanguineus. Examination of 514 dogs admitted to veterinary clinics in Egypt revealed that 67.5% were infested with R. sanguineus. Two hundred and sixty adult ticks were collected. Beauveria bassiana and Metarhizium anisopliae were then tested for their effect on these ticks. The in vitro effect of different concentrations of B. bassiana on engorged females, unfed females, fed males, eggs, larvae, and nymphs was strong for all three concentrations of B. bassiana compared with controls (P<0.05) and white fungal colonies grew on the surface of the ticks. The B1 of B. bassiana (108 conidia/ ml) was the most pathogenic on adult and developmental stages of ticks. The impact of different concentrations of M. anisopliae on adult and developmental stages in comparison with controls was similar, with the growth of green hyphae around eggs and adult ticks which prevented hatching and resulted in tick death. The M2 suspension (107 conidia/ml) had the most potent effect on adult ticks and developmental stages. The efficacy of this suspension was higher than that of the B1 concentration of B. bassiana (98 and 100% respectively). Therefore, 107conidia/ml of M. anisopliae seems to be the most effective fungus to use as bio-pesticide to control different developmental stages of R. sanguineus and may be a reasonable alternative to chemical treatment.
Keywords: Bio-pesticides, Entomopathogenic fungi, Beauveria bassiana, Metarhizium anisopliae, Rhipicephalus sanguineus
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Antidiabetic Effect of Pumpkin Seeds and Gum Arabic and/or Vildagliptin on type 2 Induced Diabetes in Male Rats
Mohamed F Dowidar, Amany I Ahmed and Hanaa R Mohamed
Abstract
Abstract
Metabolic syndrome is the accompaniment of multiple risk factors for atherosclerotic cardiovascular disease and type 2 diabetes (T2D) including glucose intolerance, hypertension and dyslipidemia where obesity is the basic element at this syndrome. T2DM is a heterogeneous metabolic syndrome resulting from genomic-environment complex interaction. Pumpkin seeds and gum Arabic are two natural products with an obvious hypoglycemic role. Vildagliptin is a selective inhibitor of dipeptidylpeptidase-4 (DPP-IV), and a certified therapy for T2DM. This study has been destined to assess in vivo the hypoglycemic impact of pumpkin seeds and gum Arabic in compare with vildagliptin and their combination treatments. 70 male albino rats were randomly separated into seven groups of ten each: control non-diabetic, control non-treated diabetic, diabetic treated with pumpkin seed extract (300mg/kg/day), diabetic treated with gum Arabic (500mg/kg/day), diabetic treated with vildagliptin (10mg/kg/day), diabetic treated with a combination of pumpkin seed extract and vildagliptin, and diabetic treated with a combination of gum Arabic and vildagliptin.T2DM was stimulated by feeding rats on high fat-high fructose/sucrose diet regimen (HFHF/S) for 12 weeks. Biochemical assessment has revealed that pumpkin seed extract, gum Arabic and vildagliptin can alleviate the severity of hyperglycemia, insulin resistance, dyslipidemia and depress glucagon secretion.
Keywords: Type 2 diabetes, Insulin resistance, Pumpkin seeds, Gum Arabic, Vildagliptin
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Salmonella Enteritidis in Broiler Chickens: Isolation, Antibiotic Resistance Phenotyping and Efficacy of Colistin on Control of Experimental Infection
Mohamed M. Amer, Aziza M. Amer, Eman R. Hassan and Aly M. Ghetas
Abstract
Abstract
Out of 400 examined samples 45 suspected Salmonella isolates (11.25%) were obtained 19 (9.5%) out of apparently healthy and 26 (13%) from diseased chickens. Intestinal samples had more isolates (29, 14.5%) more than liver (16, 8%). Identified S.Enteritidis from suspected salmonella was 16/45 (35.6%) with a rate of 8% out of the examined 400 samples, 6 (3.0%) out of apparently healthy and 10 (5.0%) from diseased chickens. Intestinal samples had more isolates (11, 5.5%) than liver (5, 2.5%). The Antibiotics susceptibility profile of S. Enteritidis isolates revealed 100% resistance to trimethoprim/sulfamethoxazole, followed by oxacillin (62.5%), 56.3% for each of ampicillin, clindamycin, enrofloxacin and doxycycline, 50% for chloramphenicol, 43.8% for streptomycin, 37.5% to cephalosporins and 18.8% for colistin. Tested S. Enteritidis isolates are classified into 11 profiles and are resistant to two – nine antibiotic classes with resistant index 0.2- 0.9. Only two isolates are NDR (12.5%), most of isolates 10/16 (62.5%) are MDR and 25% are EDR to 8-9 antibiotics. Clinical signs in experimentally infected chickens appeared at 2nd dpi, mortality started at the 4th to reach 27.5% in infected nontreated and 5% in colistin treated. Signs and lesions were markedly severe in infected nontreated than treated. S. Enteritidis was re-isolated from dead infected birds. S. Enteritidis intestinal count in sacrificed infected nontreated was higher than treated. Colistin treated group showed higher FCR, EEF and CV% (1.52, 402.8 and 6.12%) than infected non-treated (1.73, 222.6 and 14.83%). It could be concluded that S. Enteritidis is prevalent in broiler chicken flocks. Most of the isolates are MDR. Experimental infection of broiler with S. Enteritidis field isolates resulted in high mortality and the addition of colistin sulphate in drinking water controlled the infection and restores the productivity of infected broiler chickens.
Keywords: Broiler, S. Enteritidis, isolation, Multidrug resistance, Experimental infection, Colistin
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