Abstract
The equidae distal limb represents a challenge for clinicians during the diagnosis of lameness associated with this region. Diagnostic imaging modalities are essential in the diagnosis and differential diagnosis of many different causes of lameness in the distal limb of equidae. Because each type of imaging technique is superior in imaging such tissues and has its unique strengths and weaknesses, synergism between these various imaging techniques makes diagnosing distal limb lameness easier and more accurate. Moreover, the use of many imaging modalities has also enabled the equine clinician to evaluate therapy response, guide rehabilitation recommendations, and more precisely guide treatment. There are several techniques used for the diagnosis of distal limb affections in equidae such as Radiography, Ultrasonography (US), Computed tomography (CT), Magnetic resonance imaging (MRI), and Positron emission tomography (PET) scan. The present review briefly gives an update on the diagnostic imaging techniques used to diagnose the distal limb affections in equidae.
Keywords: Computed tomography, distal limb, Equidae, Magnetic resonance imaging, Positron Emission Tomography, Radiography, Ultrasonography.

Abstract
Body measurements are crucial indicators of growth performance and productivity in livestock. This study investigates the genetic basis of key body measurements in Saburai goats, a breed developed in Lampung Province, Indonesia, through grading up between Ettawa Grade does and Boer bucks. Genome-Wide Association Studies (GWAS) were employed to identify genetic markers associated with shoulder height, body length, and chest girth, which are important traits for growth and meat production. Phenotypic data from 100 Saburai goats were collected, and genotyping was conducted using the Illumina Goat SNP52 BeadChip. Statistical analyses using a mixed model identified significant genetic markers, including SNPs on chromosomes 6, 14 and 19, associated with traits such as chest girth, body length, and shoulder height. Several annotated genes, such as HDAC5 and CSN1S1, were implicated, highlighting their potential roles in growth and development. The results provide valuable insights into the genetic architecture of these traits, facilitating marker-assisted selection to enhance breeding programs. This research underscores the utility of integrating phenotypic and genotypic data to accelerate genetic improvement in livestock, offering a foundation for developing more productive and resilient Saburai goats. The findings also contribute to broader genomic studies in small ruminants, emphasizing the role of GWAS in modern livestock breeding strategies.
Keywords: Genome-wide association study (GWAS), Saburai goat, Body measurements, Genetic markers, Breeding improvement.

Abstract
The aim of our study was to determine clinical and pathological characteristics of aeromonosis in sturgeon fish in the conditions of recirculating aquaculture systems and to study the therapeutic effect of bitter wormwood. Fish with clinical signs of the disease were studied, external lesions, behaviour, and reactions were examined, pathological and anatomical analysis was performed, and the degree of lesions was assessed. To investigate the therapeutic properties, 5 experimental groups (n=20) were created. For group 1, an aqueous infusion of wormwood herb was used in the form of therapeutic baths. For group 2, an aqueous wormwood herb infusion was administered orally in therapeutic baths. Group 3 was a complex preparation. For group 4, antibiotics were administered. For group 5, table salt was used. It has been established that at recirculating aquaculture systems, aeromonosis runs in acute and subacute form with clinical signs of bacterial-hemorrhagic septicemia. More than 70% of morbidity in acute form was found mainly in fry and yearlings. In the subacute form, fish over a year old and older, less frequently fried and yearlings were predominantly affected. The study of therapeutic properties showed that a low mortality rate was observed in the 1st and 3rd groups. No fish mortality was observed in group 2. The survival rate of fish in the 1st, 2nd and 3rd groups was higher (from 90%) than in the 4th (75%) and 5th (60%). No side effects, irritation, or recurrence were observed with wormwood infusion. The results of studies may be helpful for researchers and specialists in fish farming and aquaculture.
Keywords: Aeromonas hydrophila, sturgeons, pathology, clinical sign, Artemisia absinthium L.

Abstract
This work aimed to improve the Holstein and Jersey cow HH2 and HJ1 fertility haplotype diagnostics, which indicate early embryonic mortality. Studies were conducted on 150 cows and 78 breeding bulls of the Holstein breed and 150 cows and 6 bulls of the Jersey breed. The Republic of Kazakhstan’s cow population had 5.4 and 3.4% haplotypes associated with cow fertility, respectively. Due to its low cost, speed, and restrictions exclusion, the Tetra-primer ARMS-PCR reaction is recommended for detecting heterozygous carriers of the HH2 fertility haplotype. The proposed real-time polymerase chain reaction method accurately and quickly distinguishes homozygous healthy Jersey cattle and heterozygous carriers of the HJ1 haplotype of fertility. The DNA content in the reaction mixture should not be below 15ng/µL for a reliable reaction. Periodically screening breeding stock and bulls for genetic anomalies can help control the spread of harmful mutations.
Keywords: Single nucleotide deletion, Point mutation, Polymerase chain reaction, Genetic monitoring, Cattle

Abstract
Beef is meat from cattle that is highly perishable if not stored or treated properly. Rhodotorula glutinis has antimicrobial properties, and if used with whey protein could potentially produce a coating material to preserve the beef. This research aims to determine the effect of coating materials using the antimicrobial component Rhodotorula glutinis in whey on the physicochemical, microbiological and organoleptic properties of beef. Four treatments (T0 = 100% whey, T1 = 25% whey: 75% Rhodotorula glutinis, T2 = 50% whey 50% Rhodotorula glutinis, T3 = 75% whey 25% Rhodotorula glutinis) were prepared. The cooking loss, water holding capacity, tenderness, fat, protein, moisture, initial spoilage, total plate count, resistance, and organoleptic properties analyses were carried out. The use of whey and the antimicrobial component Rhodotorula glutinis as an edible coating did not affect physical quality, chemical quality, microbiological quality and organoleptic except for T1 which had a significant effect on the tenderness value after 5 hours (63.28%), protein content (22.02%), fat content (7.99%), and initial spoilage (681min). In conclusion, using 25% whey and 75% Rhodotorula glutinis is the best concentration as a coating for beef preservation.
Keywords: Beef, Coating, Rhodotorula glutinis, quality, Whey

Abstract
Poultry farming is key to providing the population with high-quality, affordable products. The successful development of this industry is directly dependent on the rational use of available feed resources. The study aims to explore the influence of different concentrations of activated Shungite as part of compound feed on the productivity of quail and the quality and chemical composition of quail eggs. The experiment was conducted on a quail farm owned by the SalemQus LLC in Almaty region, Kazakhstan. The study subjects were three groups of quail of the meat breed Texas, 50 animals each, selected by random sampling. The control group received a standard diet without additives, while groups 2 and 3 were supplemented with activated Shungite in doses of 3 and 5%, respectively. After 120 days of the experiment, the influence of activated Shungite on the egg productivity of laying quail hens produced a significant difference. Adding activated Shungite into the diet of laying hens in 5% of their main feed improved their egg-laying indicators, increasing gross egg production by 2.82% and egg-laying rate by 98.8%. The average egg weight increased by 6.50% and egg mass yield by 9.48%. An improvement was also observed in the morphometric parameters of eggs. The phosphorus and iron content in the eggs rose by 5.1 and 7.25%. This study confirms the effectiveness of activated Shungite in poultry feeding and substantiates the need for further research to optimize the dosages and study long-term effects.
Keywords: Feed additives, Shungite, Quail, Productivity, Quality assessment, Mineral composition.

Abstract
The Pasundan breed is native cattle from West Java, Indonesia, that the farmers keep as beef cattle. This study aimed to detect the paternal lineage of Pasundan bulls inferred from the partial sex determining region Y (SRY) gene. The blood samples of Pasundan bulls (20 heads) and frozen sperm (straw) of Ongole grade bulls (10 heads) were used for DNA extraction analysis. In the present study, Pasundan and Ongole grade bulls were kept at the breeding station. The results showed that a g.15264C>T mutation point was detected in the SRY gene of animals under study. This mutation was shown as the genetic marker to classify them into Indicine and Taurine lineages. In conclusion, Pasundan cattle in this study have two paternal lineages of Bos indicus (80%) and Bos taurus (20%).
Keywords: Paternal lineage, Mutation point, Pasundan cattle, SRY gene.

Abstract
The present study is a retrospective analysis of the bovine leukemia epizootic situation in the Republic of Kazakhstan from 2019 to 2023. The study examines trends in the spread of the disease, its geographical distribution, and the effectiveness of existing control measures in Kazakhstan. The study aims to conduct a retrospective analysis of the bovine leukemia epizootic situation in Kazakhstan from 2019 to 2023, identify trends in the spread of the disease, and propose measures for its control and prevention. The work uses data from official veterinary reports and the results of serological blood tests run by local veterinary inspections covering various administrative regions of Kazakhstan. The obtained data show that, despite the preventive measures being implemented, in several regions, such as North and East Kazakhstan, the rate of leukemia infection remains higher than in others. This highlights the need for enhanced measures in high-risk areas. There was also a tendency for the infection rate to fluctuate with peaks in morbidity and subsequent decreases after recreational activities. Despite current preventive measures, bovine leukemia remains a serious problem for the Kazakh livestock industry. The study points to the need to improve the interaction between veterinary services, farm managers, and local governments to optimize disease control strategies. The importance of continuous monitoring and targeted interventions in high-morbidity areas is emphasized. The results provide valuable data for future programs to eradicate leukemia and preserve animal genetic stock and productivity.
Keywords: Leukemia, Cattle, Retrospective analysis, Republic of Kazakhstan, Epizootic situation.

Abstract
Arabian sand gazelle (Gazella marica) is listed as a vulnerable species. Few studies exist on the anatomy and histology of its reproductive system. The purpose of this work is to fill this gap by providing comprehensive characterization of female reproductive anatomy and histology and establishing baseline data for various areas of the reproductive tract. The results showed that the ovary is composed of a simple squamous to cuboidal epithelium, forming the germinal epithelium, where follicles are arranged in the cortical region of the ovary. The oviduct is characterized by a simple columnar, highly folded epithelium with decreasing diameter as it reaches its end. Peripheral gland-like structures are seen along the extension of the oviduct epithelium, more often in the infundibulum than in the isthmus. The uterus is bicornuate, similar to the bovine uterus, but with a deep groove between the uterine horns reaching the cervix. Caruncles of the uterus are pigmented and found only in uterine horns, suggesting a cotyledonary placenta. The endometrium exhibits an abundance of coiled simple tubular glands in both the uterine horns and the uterine body. Three cervical folds in the cervix exhibit incomplete encirclement that lacks a fully formed ring structure, facilitating the introduction of semen catheters. The cervical epithelium is mainly composed of secretory cells. The structure of the reproductive tract in Arabian sand gazelle is similar to that of domestic ruminants, such as cows, sheep, and goats, and wild small ruminants, with few discernible variations.
Keywords: Gazelle; Anatomy; Female reproduction; Histology; Uterus; Wildlife conservation.

Abstract
The present study was designed to assess the impact of extruded feed on laying hens using a meta-analysis approach. The database was established based on 18 articles divided into processed and supplemented extruded feed ingredients. The information was analyzed using OpenMEE software, considering the specific studies as random and extruded feed as fixed effects. The results presented no statistically significant variance in the effects of extruded feed on hen-day production, but there was a significant variance in feed conversion ratio (FCR) and feed intake (P<0.05). Regarding egg quality, there were no alterations in albumin weight, Haugh units, or yolk weight between the processed and unprocessed feed ingredients. Egg weight and albumin height (P<0.05) increased significantly with processed extruded feed ingredients. In terms of comparison of supplementation with extruded feed, the performance of laying hens was not significantly higher with supplementation than without supplementation of extruded feed. The egg quality and egg weights of laying hens fed with the supplemented diets were significantly lower than those of hens fed with the diets without supplementation (P<0.05). There was no statistically significant variance in the effects of supplementation with extruded feed on albumen weight, Haugh unit, and yolk weight. In conclusion, the effects of processing extruded feed and supplemented extruded feed ingredients differ in performance and egg quality.
Keywords: Poultry, Supplements, Extruded feed, Performance, Egg and meat quality.

Abstract
The potential therapeutic application of natural compounds, particularly aromatase blockers, in hormone-related conditions has garnered significant interest due to their ability to reduce aromatase activity with minimal adverse effects. Cruciferous vegetables produce indole-3-carbinol (I3C), an estrogen receptor (ER) antagonist and natural preventive. In particular, I3C’s role in reproductive testosterone regulation is unknown. This study examined how I3C affects male rat reproductive hormones (estrogen and testosterone). Adult male rats received oral I3C at human therapeutic levels for four weeks. Enzyme-linked immunosorbent Assay (ELISA) and immunohistochemistry (IHC) were used to assess hormone levels and receptor expression post-treatment. The I3C therapy lowered testes estrogen receptor alpha (ER-?) expression, increased testosterone receptor abundance during spermatogenesis, and boosted testosterone levels. This study shows that I3C ingestion raises testosterone levels and receptor expression, regulating testosterone. Although the findings were insignificant, they suggest an interaction between testosterone and I3C. Testosterone receptors, along with ER-? levels, vary significantly across treatment groups. For testosterone receptors, the values were: Aquadest 49.60±4.85; letrozole 55.28±3.95; dose 1 44.34±2.50; dose 2 66.31±16.39; dose 3 79.49±9.00. For ER-?, the levels were: letrozole 163.63±38.85; dose 1 217.63±30.04; dose 2 180.50±51.83; dose 3 61.25±22.30. We found that broccoli powder with an I3C component may influence male animals’ reproductive systems for the first time. Further research, including clinical trials, is needed to understand how I3C regulates reproductive hormones and its molecular pathways.
Keywords: Aromatase blocker, Testosterone and Estrogen, Indole-3-carbinol, Receptors, Reproductive hormones, Rats.

Abstract
Long bones injuries and fractures are common orthopedic problems occur in sheep and goats. This study aimed to evaluate fractures of the metacarpal and metatarsal bones in sheep and goats (clinical study) and to describe the accelerating action of silver nanoparticles Thymus vulgaris (Ag-NPs-THV) on healing process of fracture in goats (experimental study). AgNPs were reduced using THV to form AgNPs-THV. The formulated AgNPs-THV was characterized for size, charge, morphology, and the drug content of THV was determined for further experiments. Twenty-four adult male Barbary goats were randomly assigned into two equal groups. A cortical bone defect 3.5mm diameter was made at the middle third of the right metacarpal bone in each goat. Group 1 (THV Extract) and Group 2 (AgNPs-THV) were injected with THV extract liquid, and AgNPs-THV at the bone defect, respectively. The healing process was evaluated for eight weeks after surgery depending on the radiological, turnover biomarkers of bone, and histopathological assessment. Additionally, 49 sheep and goats with metacarpal and metatarsal bone fractures were included in this study on the basis of clinical, and radiographical examinations. The formulated AgNPs-THV was oval and formed as cluster. The size was in nano range, while the charge was -18.1±1.74mV. Furthermore, the recorded wavelength was 388.5?2.2nm, and THV was recorded in AgNPs-THV as 5765.336µg/mL. The radiographic union scale was significantly affected by treatment and time, P<0.001. AgNPs-THV significantly provided better radiographic union scale than that provided by THV Extract at 2,5 and 8 weeks post treatment. Histopathologically, at 5 weeks post-operative, Ag-NPs-THV treated group showed a significant decrease of fracture gap that filled with woven bone beside lamellar bone in comparison with THV Extract group (P<0.01) that still had wider gap filled with granulation tissue and limited woven bone. At 8 weeks post-treatment, 100% of cases treated with Ag-NPs-THV showed complete healing with lamellar bone compared with 50% of THV extract treated group. Ag-NPs-THV colloidal nano-formulation could be used as a promising formulation to speed up healing process of tibial bone fracture in rabbit.
Keywords: Diagnostic imaging; Pathology; Pathophysiology; Ruminant; Thymus vulgaris.

Abstract
This study aims to address the lack of comprehensive epidemiological data on Equine Rhinopneumonitis in Kazakhstan by providing a detailed analysis of the disease’s prevalence and distribution from 2021 to 2022. The purpose was to diagnose horses for the disease and implement measures for its treatment and prevention. To investigate the epidemiological situation of equine rhinopneumonitis in Kazakhstan, we collected data from three stud farms located in different regions of the country over the years 2021-2022. The sample included clinical, laboratory, and molecular-genetic data from horses exhibiting symptoms of the disease. During the period under review, the incidence of equine rhinopneumonitis increased, with five epidemiologically affected regions identified in Kazakhstan. The study found statistically significant differences in symptom prevalence among groups of horses with nasal discharge, coughing, fever, and apathy (F (3, 396) = 6.23, P=0.001). Analysis of age groups showed significant variations in morbidity rates (F(2, 297)=3.91, P=0.02). In 2022, the mean number of equine rhinopneumonitis cases was 15±5, while in 2023, it increased to 20±7. Additionally, the study revealed that the prevalence of the disease was higher in the North Kazakhstan region than in other regions. Analysis of phylogenetic data aids in understanding the virus’s origin and its relationship with other strains, which can be instrumental in developing effective strategies for controlling and preventing Equine Rhinopneumonitis.
Keywords: Buffer zone, Equine Rhinopneumonitis, Foals, Herpesviridae, Herpesviruses, Horses, Viral abortion, Viruses.

Abstract
Taenia taeniaformis is a unique tapeworm found in cats. However, limited information on this worm isolated from domestic cats has been documented, including morphological and molecular data on its adult stage. This study aimed to describe the morphological characteristics of T. taeniaformis and molecularly identify this tapeworm based on mitochondrial markers. Sixty-one intact adult worms were selected for measurement, specimen preparation, and subsequent observation of morphological characteristics of the scolex, neck, and proglottids. All tapeworms were then subjected to PCR assays to amplify partial cox1 and 12S rRNA genes. Sequencing techniques were used to sequence the two PCR products amplified from each marker. In results, the mean length of adult tapeworms was 10.57cm, with widths ranging from 0.2 to 0.45cm. The scolex possessed a rostellum armed with double rows of hooks ranging from 32 to 48. The highest frequency was observed in tapeworms, with 38 hooks, constituting 32.79%. The neck was short, the immature proglottids were narrow and elongated, the testes were scattered throughout the proglottids, the ovary and vitelline gland were bilobed, the genital pores opened unilaterally on both sides, and the gravid proglottids consisted solely of a branched uterus. Molecular analysis classified this tapeworm as T. taeniaformis. This study represents the first examination of the morphology of adult T. taeniaformis, with particular emphasis on the variation in rostellum hook numbers (32-48). Further research is necessary to assess intraspecific variation in T. taeniaformis concerning this difference.
Keywords:12S rRNA, cox1, Domestic cat, Rostellum hooks, Taenia taeniaformis

Abstract
Postoperative wound care generally uses synthetic drugs that can cause antibiotic resistance, so a safer and more effective alternative is needed. Corn hair (Zea mays L.) is an abundant waste and rich in phenolic compounds that have anti-inflammatory and wound healing activities. This study aims to develop and evaluate ointment from corn hair extract as an anti-inflammatory and wound-healing agent. Corn hair extract ointment concentrations of 5, 7, and 9% were tested in vivo using a cut wound model in mice (Mus musculus) to determine the wound healing process. In addition, hematology tests and histopathology tests of mice were conducted to see the healing response of corn hair ointment. Data were analyzed using One Way ANOVA with the IBM SPSS® 25 program. The test results showed that corn hair extract ointment with a concentration of 7% has great potential as an effective and safe natural alternative for postoperative wound care, with significant anti-inflammatory and wound healing abilities.
Keywords: Anti-inflammatory, Corn Silk, Ointment, Wound Healing.

Abstract
This study aimed to determine prevalence of nasopharyngeal myiasis among camels and evaluate the variation of Mentha longifolia oil yield, components, antioxidant activity, and larvicidal effect against Cephalopina titillator during three cuts (28th of April, 4th of June, and 22nd of July). Clinical and post-mortem examinations of 300 dromedary camels slaughtered in Giza province, Egypt showed a high prevalence of nasopharyngeal myiasis (58%). Risk factors linked to high infestation were being more than 3 years old (70.8%) and cooler seasons (74%); where it reached 81.3% in Winter. The highest oil yield was obtained on the 22nd of July. The relative percentages of the main constituents (pulegone, followed by eucalyptol, then I-menthone) showed variation in all three cuts. The highest percentage of pulegone (63.79%) was on the 28th of April, followed by 62.41% on the 4th of June, and decreased to 55.25% on the 22nd of July. In contrast, the 3rd cut produced the highest Eucalyptol (18.26%) and I-menthone (9.83%). M. longifolia oil at 200mg/mL caused 100% mortality of 2nd and 3rd larvae within 2 and 8h, respectively. Essential oil of the 2nd cut showed superior efficacy compared to ivermectin-treated larvae, with lower LC50 and LC90 values. Light and scanning electron microscopy revealed significant damage to the oil treated larvae, including swelling, degeneration, and atrophy of the cuticle, the mouthparts and antennal lobes. These findings highlighted the danger of nasopharyngeal myiasis, effect of cut dates on M. longifolia oil yield and constituents, and its potent anti-C. titillator.
Keywords:Camel nasopharyngeal myiasis, Cephalopina titillator, Mentha longifolia, Pulegone, Cuts dates.

Abstract
Nakhchivan Autonomous Republic (NAR) is a region known for its rich biodiversity and traditional use of herbal medicine for animal health. Ethnobotanical studies were conducted in seven districts, and interviews with 162 farmers (108 females and 54 males) documented the traditional use of 14 medicinal plants for the treatment of various animal diseases. The findings highlight the wound-healing and antiparasitic effects of Achillea millefolium (yarrow), the antiseptic and anti-inflammatory properties of Hypericum perforatum (St. John’s wort), and the widespread veterinary applications of Plantago major (plantain) for respiratory and digestive disorders. Artemisia absinthium (wormwood) and Equisetum arvense (horsetail) were also identified for their antiparasitic and diuretic properties, respectively. Modern pharmacological studies confirm the presence of bioactive compounds, such as flavonoids, phenolic acids, and essential oils that support many traditional uses. The results highlight the therapeutic potential of these plants by establishing a strong link between ethnoveterinary knowledge and scientific evidence. However, this study also highlights the need for further scientific validation, including phytochemical analyses and clinical trials, to standardize dosages and ensure safe veterinary practices. Bridging traditional knowledge with modern science, this research highlights the potential of the NAR flora in developing sustainable veterinary treatments.
Keywords: Ethnobotany, Medicinal plants, Animal health, Nakhchivan Autonomous Republic flora.

Abstract
Livestock productivity can decrease due to paramphistomosis caused by Paramphistomum spp. This study aimed to determine the prevalence of the disease, analyze the risk factors, and present a distribution map of paramphistomosis in beef cattle in Manokwari Regency, West Papua. Using simple random sampling, a total of 1336 beef cattle fecal samples were collected from 206 smallholder farms in seven districts in Manokwari Regency from December 2023 to February 2024. Risk factor analysis was conducted to see associations with disease incidence and GIS maps were created to visualize the prevalence distribution of paramphistomosis in Manokwari regions. The overall prevalence of paramphistomosis was 45.56%, while at the farm level, it was 75.73%. The sampling location, livestock origin, feeding method, drinking water sources, and farmers’ education level were risk factors that correlated (P<0.05) with the prevalence of Paramphistomum spp. infection in beef cattle. Manokwari Selatan District was detected as an area with a high prevalence of paramphistomosis. Based on the epidemiological data, it can be concluded that the level of endemism of paramphistomosis is quite high in Manokwari Regency, West Papua. The results of this study reveal the role of livestock management systems and farmer education factors in influencing the geographic heterogeneity of the prevalence of beef cattle paramphistomosis. These risk factors can be used to develop paramphistomosis control strategies, farmers are adequately educated about health problems of livestock that these parasitic infections cause to their livestock productivity.
Keywords:Beef cattle, Distribution, Epidemiology, Manokwari, Paramphistomosis.

Abstract
This study aimed to investigate the effectiveness of microencapsulating plant-derived essential oils (EOs) that are rich in total polyphenolic content (TPC), flavonoid content and antioxidant capacity. Specifically, the microencapsulated garlic essential oil (mGEO) and microencapsulated lemongrass essential oil (mLEO) formulated with black soldier fly-based protein (BSF) extract in oil phase solution using spray-drying technique. The results demonstrated the encapsulation process positively influenced the phytochemical characteristics, particle size, zeta potential, and polydispersity index (PDI) of the microcapsules, resulting in an encapsulation efficiency of 84.7 and 20.2%, respectively. The mGEO microcapsules had the highest TPC released in vitro after 24h in simulated rumen pH 5.6 (45.0%) and small intestine pH 7.4 (93.3%) buffers when compared with the mLEO microcapsules, which were 50.0 and 77.8%, respectively. Additionally, the released TPC from EO capsules demonstrated in vitro antimicrobial activity, particularly the mGEO microcapsules, which exhibited the antimicrobial efficacy against animal pathogens, specifically mastitis-causing bacteria such as Escherichia coli, Enterobacter aerogenes, Shigella typhimurium, Staphylococcus aureus, and Bacillus cereus. The result of this investigation indicated that the MIC and MBC values were below 100µg/mL, comparable to the concentration of the positive control (e.g., monensin). Moreover, active substance as allicin was predicted and confirmed in terms of bioactive molecule, eco-friendly, in silico pharmacokinetic and toxicological properties. This study suggests that microencapsulation technology of phytochemical-derived EOs has a promising future as feed additive for controlling release and combat animal pathogens.
Keywords: Microencapsulated additive; Essential oils-feeding; In vitro release; In vitro antimicrobials; In silico ADME, Molecular docking.

Abstract
The current research conducted on the 500 camels in the Bahawalpur district of Punjab, Pakistan, aimed to examine the presence of trypanosome flagellate protozoan parasites and to determine the alterations in the hemato-biochemical parameters of diseased camels in the Bahawalpur district. The collected blood samples were immediately used to prepare fresh thin blood smears on glass slides, air-dried, and then fixed with absolute methanol. The smears were subsequently stained with Giemsa stain, and trypanosomes were identified microscopically. Different physiological parameters like temperature, heart beat and breathing rate, along with other clinical signs including depression, anorexia, dehydration, lethargy, and edema of pads were also recorded. The results revealed a significant increase (P<0.05) in white blood cells (WBCs), including neutrophils, basophils, eosinophils, and monocytes, as well as in mean corpuscular hemoglobin concentration (MCHC) in the infected camels. However, the study observed a significant reduction (P<0.05) in the hemoglobin concentration, lymphocyte count, hematocrit, and red blood cell (RBC) count. The blood biochemical parameters, like aspartate aminotransferase (AST), alkaline phosphatase (ALP), and alanine aminotransferase (ALT), were found to be significantly elevated (P<0.05) in the infected camels. While serum albumin, total proteins, calcium, magnesium, and phosphorus were significantly lowered (P<0.05) in the diseased camels compared to the healthy ones. However, levels of serum lipid peroxidation product (malondialdehyde) were significantly increased (P<0.05) in the infected camels. The microscopic examination of blood smears from infected camels revealed the presence of trypanosome parasites along with various morphological changes in the erythrocytes.
Keywords:Diseased Camels, Blood samples, Microscopic examination, Giemsa stain, Trypanosome parasites

Abstract
The objective of this study was to investigate the effects of different factors on the first service conception rate (CR) in Dansk Holstein cows raised on a farm in the Southern Denmark. Data including age of cows, parity, age at the first service (AFS), age at the first calving (AFC), calf’s gender, previous gestation length, calving to service interval (CSI), calving season, and service season of 699 cows was collected from the herd’s database. Logistic regression was used to evaluate the effects of the investigated factors on the CR. Results showed that AFS, AFC, calving interval, CSI, and CR were 410.6±22.2, 714.5±50.2, 394.5±59.7, 87.6±23.7 days, and 40.8%, respectively. The odds of conception in parity 3 (OR=0.41, 95%CI=0.26-0.64, P<0.001) was lower than that in parity 1. A CSI of 36-60 days (OR=0.28, 95%CI=0.16-0.48, P<0.001) and 60-80 days (OR=0.50, 95%CI=0.29-0.85, P<0.01) resulted in a lower CR than a CSI of 81-90 days. Cows calving in spring (OR=3.59, 95%CI=1.76-7.33, P<0.01), summer (OR=3.24, 95% CI=1.64-6.42, P<0.01), and winter (OR=3.89, 95% CI=2.07-7.30, P<0.001) had a higher CR than in autumn. Cows having service in spring (OR=3.49, 95% CI=2.16-5.63, P<0.001), summer (OR=3.29, 95%CI=2.08-5.19, P<0.001), and autumn (OR=3.79, 95%CI=2.23-6.43, P<0.001) had a higher CR than in winter. An AFC of 671-700, 701-730, and >760 days (OR=0.34-0.52, P<0.05) conferred a lower CR than that of 646–670 days. This study indicates that parity >2, AFC >670 days, CSI <60 days, calving in autumn, and service in winter are risk factors for reduced CR in the investigated Dansk Holstein cows.
Keywords: Age, Conception rate, Holstein cows, Reproduction, Season.

Abstract
This study aimed to characterize the seminal plasma protein profiles of Kokok Balenggek roosters and investigate their relationship with semen quality parameters. Semen samples were collected from 15 roosters and analyzed for motility, viability, abnormality, and intact plasma membrane (IPM). Seminal plasma proteins were separated by SDS-PAGE, and molecular weights were estimated. The results showed that the 51kDa protein band exhibited strong positive correlations with sperm motility, viability, and IPM, and a significant negative correlation with sperm abnormality. These findings suggest that the 51kDa protein, possibly clusterin, plays a crucial role in sperm function and may serve as a potential fertility biomarker in indigenous poultry. The study highlights the importance of seminal plasma protein profiling in improving reproductive performance and selection strategies for local poultry breeds. Further studies are recommended to identify the specific proteins involved and their biological functions.
Keywords:Kokok Balenggek roosters, SDS-PAGE, Seminal plasma proteins, Sperm quality

Abstract
Clinical signs of respiratory infection are observed annually among the horse population of the Kyrgyz Republic. In this regard, we collected 289 blood serum samples from horses and tested them using the enzyme-linked immunosorbent assay (ELISA) for the presence of antibodies against equine influenza virus (EIV) and equine herpesviruses type 1 and 4 (EHV-1 and EHV-4). The results of the studies showed that virus-specific antibodies against EHV-4 were detected in all collected blood serum samples. Antibodies against EIV were detected in 54% of the tested samples, while only 3.9% tested positive for EHV-1. These results can serve as fundamental data for preventing the spread of equine infectious diseases in Kyrgyz Republic in the future.
Keywords: EIV, EHV, ELISA, Horse, Kyrgyz Republic, Serological Monitoring.

Abstract
The fermentation of durian fruit waste using Lentinus edodes can reduce the crude fiber content while increasing the protein content. This study aims to evaluate the impact of incorporating fermented durian waste (FDW) with Lentinus edodes into the diet on the production performance and egg yolk quality of quails. This research utilized 200 quails that were 40 days old. The design employed is an entirely randomized design featuring 5 treatments and 4 replications, with each replication comprising 10 laying quails. The treatments administered included A (0% DzWF), B (3% DzWF), C (6% DzWF), D (9% DzWF), and E (12% DzWF) in the quail meal. The criteria evaluated encompass feed consumption, egg production, egg weight, egg mass, feed conversion, and the quality of quail egg yolks, specifically cholesterol, fat, and omega-3, -6, and -9 fatty acids (3, 6, 9, DHA). The analysis of variance results indicates that the incorporation of fermented durian waste (DzWF) in the feed does not significantly influence (P>0.05) feed consumption, egg production, egg weight, egg mass, feed conversion, quail egg yolk fat, or omega 3, 6, 9, and DHA fatty acids in quail egg yolk; however, it exerts a highly significant effect (P<0.01) on quail egg yolk cholesterol. The study concludes that incorporating up to 12% fermented durian waste (DzWF) with Lentinus edodes can sustain the performance and quality of quail eggs.
Keywords:Lentinus edodes, Fermented durian byproduct, Performance, Egg quality, Quail.

Abstract
This study evaluated the effect of adding seaweed meal (Gracilaria sp.) and gambir leaf extract (Uncaria gambir) as feed additives on nutrient digestibility, rumen fermentation products, and microbial population in vitro. Treatments comprised three levels of seaweed addition (0, 5, 10%) and three levels of gambir leaf extract (0, 0.7, 1.4%). The results showed that adding seaweed could significantly increase the digestibility of dry matter from 59.05 to 66.95% and the digestibility of organic matter from 58.92 to 68.72% (P<0.05). VFA concentration also increased, while methane production decreased from 20.19mL/g to 14.79mL/g. The addition of gambier leaf extract contributed to stabilizing the rumen pH and suppressing the protozoa population to 7.50x104cells/mL. The best combination was obtained by treating 10% seaweed and 1.4% gambier leaf extract, which showed a synergistic effect in improving rumen fermentation and reducing methane emissions. This study indicates that the combination of seaweed meal and gambir leaf extract has the potential to be an effective natural ingredient-based feed additive to improve livestock production efficiency while supporting the mitigation of greenhouse gas emissions.
Keywords: Digestibility, Gambir leaves, Methane, Protozoa, Rumen fermentation, Seaweed

Abstract
In the presence of clinical signs of disorders of the gastrointestinal tract, apteriosis and alopecia, excessive growth of microorganisms was established from the intestinal contents of birds. The article presents data on the dynamics of biofilm-forming microorganisms, Salmonella typhimurium and Candida albicans, identified in diseases of the digestive system of Psittaciformes. With a decrease in the compensatory mechanisms of mucocilliary clearance and colonization resistance of the organism due to the representation of quorum-sensing signaling molecules, an excessive growth of pathogenic microorganisms is observed. Population immobilization of the architectonics of a mature three-dimensional biofilm is mediated by intensive cell proliferation and the synthesis of exocellular molecules of the intercellular matrix. The orderliness and compactness of the multicellular heteromorphic structure of polyspecies biofilms depend on the degree of differentiation of the adhesive basal layer of a dense network of blastoconidia, hyphal and pseudohyphal forms, and gram-negative bacteria. Depending on the microorganisms’ cultivation time, a gradual increase in the absolute optical density values of the tested specimens was established. When cultured for 18, 24, 48, 72, 96, 120, and 144 hours, the intensity of biofilm formation ranged from ?0.1 to ?0.4.
Keywords:Psittaciformes; Diseases; Digestive System; Quorum Sensing; Biofilms; Densitometry; Optical Microscopy; Luminescence Microscopy; Scanning Electron Microscopy

Abstract
The use of Hermetia illucens larvae meal as a constituent of a concentrate supplement in the feeding of dairy cows in the northern Peruvian Andes was evaluated. Eight second and third calving Holstein cows were selected in the second third of lactation (between 100 – 200 days), fed on ryegrass (Lolium multiflorum L.) – white clover (Trifolium repens) pastures. Treatments were distributed in a repeated Latin square design using different levels of soybean cake and Hermetia illucens L. larvae meal, rich in protein and fat. No significant differences (P>0.05) were evident between treatments in terms of daily milk production, chemical composition in terms of total solids, protein, fat, and lactose. The cows’ hematological parameters and blood metabolites remained within reference values, indicating that the larval meal diet met protein and energy requirements. In economic terms, the total replacement of soybean cake with grub meal decreased the cost of concentrate feed. Hermetia illucens larval meal should be considered as a sustainable option to improve productive and economic efficiency in dairy cattle systems, without compromising animal health and productivity.
Keywords: Dairy production, Protein concentrate, Livestock sustainability, Pasture feeding.

Abstract
This study aimed to assess the backfat thickness (BFT) of sows and its relationship with weaning performance in one sow farm in Eastern Hungary. A total of 196 sows of different parities (P) 1-7 were investigated. Variables analyzed included BFT D109 of gestation, total born (liveborn LB, stillborn SB and mummies M), BFT D28 (at weaning), and number of piglets weaned (NwP). Test for normality was performed using One-Sample Kolmogorov-Smirnov (KS) test. Data were analyzed using Kruskal-Wallis (KW) H-test to determine if there were significant differences across parities followed by Post-hoc pairwise multiple comparisons where significance was detected. Spearman’s correlation was used to assess the relationship between BFT D28 and NwP. With a degree of freedom of 6, the results showed that parity significantly differed for BFT D109 (KW=24.114, P<0.001) and for NwP (KW=42.843, P<0.001). There were no parity differences in the LB, SB, M and BFT D28 (KW=8.642, P=0.195, KW=10.137, P=0.119, KW =14.465, P=0.025, and KW=15.371, P=0.018) respectively. Post-hoc pairwise comparisons revealed that P1 sows had a higher (P<0.001) BFT D109 than P3 and P4 sows. Sows lost 4.24mm of backfat thickness at weaning. The NwP did not differ by parity (P>0.001). Spearman correlation indicated a non-significant (P>0.05) relationship between NwP and BFT D28. The coefficient of determinant (R2=0.0437) indicated that 4.37% of variation in backfat loss during lactation can be attributed to number of weaned piglets. In conclusion, regular monitoring of backfat makes it possible to guide sow management. It could enhance sow health, fertility and productivity.
Keywords:Backfat measurement; AnyScan BF device; Hypor sow; Large-scale pig farm; Hungary

Abstract
Flies spread infectious and invasive diseases, disturb farm animals, and reduce their productivity during the summer pasture grazing. The study aims to investigate the species composition and test the effectiveness of insecticides and repellents against zoophilic flies in cattle on livestock farms and pastures in the Kostanay Region, North Kazakhstan. The research subject was Holstein cattle kept in feedlots of two types: open, where animals were kept in open enclosures, and semi-closed, where cattle stayed in enclosures equipped with sheds, in summer camps, or enclosures near cowsheds. The effectiveness of insecticides and a repellent preparation was tested to determine the duration of cattle protection in the Karabalyk, Mendykara and Sarykol Districts of the Kostanay Region of Kazakhstan. Experiments were conducted to determine the comparative effectiveness of insecticides against zoophilic flies in cattle. Delcid® and Cyperil® insecticides were applied in different concentrations to assess their insecticidal effectiveness, along with Oxarep® repellent. The use of synthetic pyrethroid insecticides reduced milk productivity losses caused by zoophilic fly attacks on cows, which had previously reached 13.68%. Spraying the cows with Delcid®, Cyperil®, and Oxarep® during the pasture period to protect against zoophilic flies led to increased milk production in livestock farms in the Kostanay Region. Each cow produced an additional 0.23L (1.45%) of milk, reducing losses from insect attacks by more than half. Among the tested agents, Delcid® demonstrated the highest insecticidal effectiveness, maintaining strong protective action over 72 hours. Cyperil® and the Oxarep® repellent were also effective, though with slightly lower and shorter-lasting activity.
Keywords: Zoophilic flies, Cattle, Pest infestation, Productivity, Synthetic pyrethroids, Effectiveness.

Abstract
Calves are highly susceptible to endoparasitic infections, which can hinder their growth. One preventive measure that can be taken is the addition of feed additives to their daily diet, which at this stage consists of milk. This study aimed to evaluate the effect of milk type on blood and gastrointestinal endoparasite infestations in calves. A total of 15 four-week-old calves were used in this study and divided into three groups based on the kind of milk provided: LK milk, commercial milk, and fresh milk. Observations were conducted on days 1, 15, and 30. Hemoparasites were analyzed microscopically using blood smear preparations, while gastrointestinal endoparasites were examined qualitatively and quantitatively using the flotation and sedimentation methods. The results indicated that no hemoparasites were detected in any of the milk treatment groups throughout the observation period. Similarly, nematode, trematode, and cestode eggs were not found in any milk groups at different times. However, oocysts were first identified on day 15 in all groups and showed a non-significant (P>0.05) decrease on day 30. Furthermore, the number of oocysts found on day 15 did not differ significantly (P>0.05) from day 30 in all milk groups. Thus, the type of milk provided over 30 days did not affect the presence of hemoparasite or gastrointestinal endoparasites in calves.
Keywords:Calves, Eimeria, Gastrointestinal worm, Hemoparasites, Milk.

Abstract
This research explores how synbiotics obtained from Tomi-Tomi fruit extract and Lactobacillus plantarum influence the growth rate, intestinal microbiome, and blood characteristics of broiler chickens. The synbiotics were prepared by incubating the Tomi-Tomi fruit extract with L. plantarum for 48 hours prior to experimentation. A group of 200 Lohmann broiler chicks was raised from day 0 until day 7, starting at an average weight of 142±2.34g. On the eighth day, the chicks were distributed into five treatment categories randomly, with each category containing five replications: CONT (control diet without synbiotics), TFE0.25 (0.25% synbiotics), TFE0.50 (0.50% synbiotics), TFE0.75 (0.75% synbiotics), and TFE1.00 (1% synbiotics). During the duration of the experiment, marked advancements were observed in weight gain, consumption of feed, feed conversion efficiency, hemoglobin levels, and mean corpuscular volume (MCV) metrics (P<0.05). Furthermore, a significant drop in pH levels in both the ileum and cecum was evident, alongside a rise in lactic acid bacteria, and a significant decline (P<0.05) in coliform bacteria in these segments of the intestine. These findings suggest that the use of these synbiotics enhances feed efficiency, supports better physiological health, and suppresses harmful microbial growth in the digestive systems of broiler chickens.
Keywords: Synbiotics, Tomi-Tomi fruit, Performance, Gut microbiota, Blood profile, L plantarum.

Abstract
Tuberculosis (TB) remains a major public health concern in Indonesia, which ranks second globally in TB burden. Zoonotic transmission involving dairy cattle has received limited attention, especially in South Sulawesi Province. This study explored the presence of Mycobacterium tuberculosis complex (MTBC) DNA in raw milk samples and examined possible transmission between humans and dairy cows in Cendana Sub-district, Enrekang Regency. A cross-sectional design was employed, involving 50 respondents, including dairy cattle owners and individuals with a documented or suspected history of TB. Milk samples (n=60) were collected from 20 farms and analyzed using PCR targeting the IS6110 sequence. Results showed that 47% of milk samples and 65% of farms were PCR-positive for MTBC. Notably, three farmers reported prolonged cough without an official TB diagnosis; two of them owned cows with PCR-positive milk. These findings highlight potential underreporting of TB in humans and possible zoonotic and reverse zoonotic transmission or contamination. This study underscores the need for integrated One Health surveillance and stronger biosecurity and milk hygiene measures in dairy farming communities.
Keywords:Mycobacterium tuberculosis, Bovine tuberculosis, Dairy cows, Epidemiology, Indonesia

Abstract
The main objective of this study was to assess the impact of local zeolite on the amino acid and fatty acid profiles in the meat of African catfish (Clarias gariepinus). The fish were divided into three groups: the first (control) group was fed a basic diet, the second (experimental) group received the same diet with 3% zeolite, and the third (experimental) group received the same diet with 5% zeolite. Sample collection and laboratory analysis followed regulatory guidelines. The results revealed positive effects of zeolite on the total amino acid content in the meat. Glutamic acid content increased by 0.5%, and methionine levels rose by 10% in the third group compared to the control group. The protein’s chemical index also improved, indicating an increase in its biological value. Regarding fatty acids, zeolite had a minimal impact. Saturated fatty acid (SFA) content increased by 3.3% in the group with 3% zeolite (31.0±0.31g/100g) compared to the control group (30.0±0.21g/100g). Omega-3 fatty acid content remained unchanged in both the control and the 5% zeolite group (8.0±0.08g/100g), but decreased by 6.3% in the 3% zeolite group (7.5±0.02g/100g). Omega-6 fatty acids increased by 8.3% in the 3% zeolite group (13.0±1.12g/100g) compared with the control group (12.0±0.12g/100g). This study demonstrates that local zeolite improves the amino acid composition and nutritional value of protein, while helping to preserve healthy fatty acids in fish, offering new possibilities for its use in aquaculture.
Keywords: African ?atfish (Clarias Gariepinus), Amino ?cids, Fatty ?cids, Zeolite, Feed additives.

Abstract
Soymilk waste has potential to be used as feed due to high nutritional value but its utilization is limited by the content of cellulose and phytic acid. To overcome this limitation, fermentation using Bacillus subtilis, a cellulase and phytase producing bacteria was carried out. This study aims to obtain the best fermentation substrate composition and fermentation time that can improve the quality and nutritional content of fermented soy milk waste (FSMW). The substrate was a mixture of Soymilk waste (SW) with cassava leaves (CL), Indigofera leaves (IL) and white leadtree leaves (WLL). The research method used a completely randomized design (CRD) with a 3×3 factorial pattern with 3 replications. Factor A was the substrate of type: A1(80% SW +20% CL), A2(80% SW+20%IL) and A3(80%SW+20WLL). Factor B was the time of fermentation: B1 (2 days), B2 (4 days), and B3 (6 days). The observed variables were protease activity, cellulose activity, crude protein content, crude fiber and nitrogen retention, crude fiber digestibility. The results showed that there was a very significant interaction (P<0.01) between substrate type and fermentation time on cellulose activity, protease activity, crude protein content, crude fiber, digestibility of crude fiber and nitrogen retention. It can be concluded that type of substrate 80%SW+20%CL and a fermentation time of 4 days gave the best results in terms of phytase activity 16.34U/mL, cellulase activity 14.61U/mL, protease 32.95U/mL, phytate content 0.33U/mL, crude fiber 7.04%, crude protein content 29.78%, and nitrogen retention 57.45%, crude fiber digestibility 57.95%.
Keywords: B. subtilis, Soymilk waste, Fermentation, Type of substrates, Feed

Abstract
A significant decrease in the level of matrix metalloproteinase-2 (MMP-2) was observed in the placental structures of ruminant animals with placental insufficiency, complicated by eclampsia in sheep and birth pathology in cows. Research findings indicated the presence of MMP-2 biomarkers solely in the degrading crypts from the maternal placental section, as well as in the vessel regions of the fetal placenta. In the tissues of the maternal portion of the placenta in sheep and cows with placental insufficiency and pregnancy complications, degradation of the connective tissue matrix of the caruncle crypts was observed, indicating a loss of the extracellular substance structure of the uterine crypts within the caruncles. Translocation of MMP-2 was observed between the villi of the chorioallantoic of the cotyledon and the epithelium of the caruncle crypts. During normal pregnancy and childbirth, MMP-2 was evenly distributed throughout the chorioallantoic cells in the fetal portion of the placenta, except for giant binucleate and hybrid trinucleate cells. Data show that matrix metalloproteinase activity increases in the caruncles and cotyledons of cows and sheep during placental insufficiency, as it degrades maternal placental extracellular matrix components, leading to reproductive problems later. The absence or decreased expression of matrix metalloproteinases may be associated with neonatal diseases in newborns.
Keywords: Ruminants; Placenta; Pregnancy; Pathology of childbirth; MMP, TIMP

Abstract
This study investigated the association between plasma testosterone concentration and semen quality parameters in Kokok Balenggek roosters (KBR), a native Indonesian breed renowned for its distinctive crowing. Fifteen roosters aged 1–2 years were used, and blood and semen samples were collected four times at 10-day intervals, yielding 60 total samples. Plasma testosterone concentrations were measured using a competitive. At the same time, semen quality was evaluated through macroscopic (volume, pH, color, odor, enzyme-linked immunosorbent assay (ELISA). In contrast, semen quality was evaluated through both macroscopic (volume, pH, color, odor, and consistency) and microscopic (mass movement, motility, viability, abnormality, and sperm concentration) assessments. The mean plasma testosterone concentration was 18.90±7.27ng/mL. Semen characteristics showed an average volume of 292.75±143.76µL, pH of 6.96±0.10, motility of 82.77±7.04%, viability of 88.94±1.62%, abnormality of 9.02±2.86%, and sperm concentration of 2630±696.42×10?cells/mL. Plasma testosterone exhibited weak and non-significant correlations with semen volume (r=–0.369), pH (r=0.400), mass movement (r=0.081), motility (r=0.075), viability (r=0.025), sperm concentration (r=0.134), and abnormality (r=–0.267). These findings suggest that plasma testosterone concentration alone does not strongly influence semen quality in Kokok Balenggek roosters, highlighting the need to consider additional physiological and environmental factors for optimizing reproductive performance.
Keywords: Kokok Balenggek rooster, Plasma testosterone, Semen evaluation, Semen quality, Sperm motility

Abstract
This research was conducted to assess the?in-vitro rumen fermentability of sorghum combined with different legumes as energy and protein sources. Treatments consisted of eight combinations of sorghum and local legumes with two proportion levels (20 and 40%), analyzed for VFA, NH3, pH, nutrient digestibility, protozoa population, gas production, and microbial protein synthesis. Results showed that 60% sorghum and 40% Indigofera zollingeriana (P5) gave the best dry and organic matter digestibility, fiber fraction, and VFA and NH3 production results. The combination of 60% sorghum and 40% Calliandra calothyrsus (P8) produced the highest microbial protein synthesis. However, no significant variations (P>0.05) were observed in protozoa population, total gas, and methane gas production across the treatments. This study concludes that certain sorghum-leguminous combinations can increase nutrient efficiency without increasing rumen gas emissions, making it a local feed alternative that has the potential to support an economical and sustainable ruminant farming system.
Keywords: Feed efficiency, In-vitro, Leguminous, Ruminant, Sorghum.

Abstract
Heavy metal contamination in aquatic ecosystems poses a severe threat to aquatic organisms, disrupting their physiological and biochemical functions. This study evaluates the impact of aqueous nickel (Ni) and cadmium (Cd) co-exposure on oxidative stress, antioxidant defense mechanisms, histological alterations, and metal bioaccumulation in the brain and muscle tissues of Tilapia fish. Fish were divided into four groups: Group A (Control), Group B (Ni exposure), Group C (Cd exposure), and Group D (Ni-Cd co-exposure) and exposed to metals for a specific duration. Oxidative stress biomarkers including thiobarbituric acid reactive substances (TBARS) and reactive oxygen species (ROS), were measured alongside antioxidant enzyme activities such as superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH). The findings revealed a significant increase in TBARS and ROS levels, indicating oxidative stress, while SOD, CAT, and GSH levels declined, suggesting impaired antioxidant defense. Histopathological analysis showed severe damage in both brain and muscle tissues, including neuronal degeneration, vacuolization, muscle fiber atrophy, and inflammatory responses, with the most pronounced alterations observed in the Ni-Cd co-exposure group. Metal bioaccumulation analysis revealed higher Ni and Cd concentrations in the brain compared to the muscle, with the highest accumulation observed in the combined exposure group. These results highlight the toxic effects of Ni and Cd co-exposure in aquatic organisms, emphasizing the urgent need for stricter pollution control measures to protect aquatic ecosystems.
Keywords: Nickel, Cadmium, Tilapia fish, Oxidative stress, Antioxidant enzymes, GSH, Histopathology.

Abstract
DLA (Dog Leukocyte Antigen), which serves as the canine counterpart to the human major histocompatibility complex (HLA), plays a pivotal role in immune response and represents a key marker of genetic diversity. This study presents the first comprehensive analysis of class II DLA gene polymorphism (DRB1, DQA1, DQB1) in four Russian dog breeds: the Samoyed, Siberian Husky, Black Russian Terrier, and Boxer. Molecular genetic methods revealed significant interbreed differences in allelic diversity. Notably, Samoyeds and Black Russian Terriers exhibited genetic conservation in DRB1 and DQA1, while maintaining pronounced polymorphism in DQB1. In contrast, Siberian Huskies and Boxers demonstrated substantial genetic homogeneity across all studied loci, except for DRB1 in Siberian Huskies. These findings characterize class II DLA genes as highly polymorphic, enabling a comparison of allele frequencies for DRB1, DQA1, and DQB1 among Russian-bred dogs. Analysis of DLA class II gene polymorphism (DRB1, DQA1, DQB1) provides the first step in characterizing existing breed diversity using Russian-bred Samoyeds, Siberian Huskies, Black Russian Terriers, and Boxers as models. The obtained data may facilitate the prediction of breed-specific predisposition to autoimmune diseases and carcinogenesis, taking into account known associations between specific haplotypes and particular diseases.
Keywords: Immune system, Major histocompatibility complex (MHC), HLA, DLA, Dogs, Autoimmune diseases, Gene polymorphism.

Abstract
Pneumonic mannheimiosis is one of the most important diseases of small ruminants worldwide, in both developed and developing countries. However, limited research activities on this disease were carried out in the developing countries of Asia and Africa, despite being home to the highest numbers of sheep and goats in the world. A literature review on pneumonic mannheimiosis in Asia and Africa reveals that most countries reported the presence of this disease, involving between 25% and 60% of sheep and goats. Mannheimia haemolytica is more frequently isolated from sheep and goats than Pasteurella multocida. Almost all countries in Asia and Africa reported M. haemolytica serotype A2 as the most common isolate, followed by serotypes A6, A1, and A7, while P. multocida biotype A is the most frequently isolated Pasteurella species. However, only a few countries practice vaccination against this disease, mostly due to the unavailability of vaccines and the ineffectiveness of most commercially available vaccines. Therefore, developing multivalent or broad-spectrum vaccines that could protect against common serotypes of M. haemolytica or that protect against both Mannhemia and Pasteurella should be prioritized.
Keywords: Pneumonic mannheimiosis, Prevalence, Vaccine development, Asia, Africa

Abstract
This study evaluated the efficacy of IntelliBond copper (Cu) and zinc (Zn) as potential replacements for antibiotic growth promoters (AGPs), focusing on their impact on growth performance, carcass yield, and gut health of broiler chickens. A total of 1,088 Cobb broiler chicks were assigned to two treatment groups: T1: inorganic (sulfate) minerals (INO), T2: IntelliBond (hydroxy chloride) minerals (IB). Over 35 days, various parameters were assessed, including performance parameters, gut morphology, carcass yield, litter quality, and mineral deposition in the liver. The results showed that IntelliBond supplementation (T2) significantly reduced (P<0.05) mortality rates from 4.96% (T1) to 3.31%, and improved litter quality, as reflected by lower moisture content and a reduction in cases of footpad dermatitis. While T2 showed slightly lower cumulative feed intake and body weight compared to T1, it significantly increased (P<0.05) the villus height and surface area by the third week. T2 did not affect carcass yield percentage, despite lower breast meat weight (P<0.05). Liver mineral analysis revealed that IntelliBond Zn deposition was slightly lower than sulfate Zn but maintained adequate levels. Despite challenges such as high ambient temperature and ammonia levels, IntelliBond Cu and Zn maintained growth performance and enhanced nutrient absorption during the finisher phase. Additionally, IntelliBond minerals contributed to a lower incidence of footpad dermatitis, indicating improved welfare outcomes. This study highlights the potential of hydroxychloride minerals as a sustainable alternative to inorganic mineral sources in broiler production.
Keywords: Broiler chickens, Carcass yield, Copper, Growth performance, Gut health, Zinc

Abstract
This study aimed to evaluate the effectiveness of four different extenders—Beltsville Poultry Semen Extender (BPSE), Phosphate-Buffered Saline (PBS), Ringer’s Lactate (RL), and 0.9% Sodium Chloride (NaCl)—on the longevity and quality of Muscovy duck (Cairina moschata) semen stored at 5°C. Semen was collected from four mature, fertile drakes and evaluated for macroscopic and microscopic quality prior to treatment. Pooled semen was diluted to a concentration of 200×10? spermatozoa/mL in each extender and stored for 72 hours. Semen quality was assessed at 0, 12, 24, 36, 48, 60, and 72 hours based on motility, viability, abnormality, and plasma membrane integrity (PMI). The results revealed that RL and BPSE were significantly more effective than PBS and NaCl in preserving sperm quality across all parameters. RL achieved the highest sperm longevity (50.4 hours), followed by PBS and NaCl (45.6 hours), while BPSE showed the shortest longevity (33.6 hours). RL also preserved the highest motility and viability and exhibited the lowest abnormality rate and best PMI over time. The findings suggest that Ringer’s Lactate and BPSE are promising extenders for short-term storage of Muscovy duck semen and may serve as effective bases for further extender optimization in avian artificial insemination programs.
Keywords: Muscovy duck, Semen extender, Artificial insemination, Sperm preservation.

Abstract
Otodectes cynotis, commonly referred to as the ear mite, is an obligate surface-dwelling ectoparasite that predominantly colonizes the external auditory canal of cats, dogs, and other carnivorous hosts, where it induces otitis externa. This study aimed to evaluate the viability of ear mites treated with herbal extracts under laboratory conditions. In the experiment, O. cynotis were collected from naturally infested animals, grouped by life cycle stages, and subjected to various environments, including sterile petri dishes as a control, and dishes treated with herbal agents such as Eupatorium fortunei, Rosmarinus officinalis, and Derris elliptica. The results of testing the developmental stages of ear mites showed that, under laboratory conditions, the maximum survival time of mites was 6 days; by the 7th day, all mites had died. In the E. fortunei extract environment, most mites killed within the first 6 hours and were completely dead within 24 hours. When tested with the Derris elliptica extract, ear mites died completely before the first 48 hours; very few mites survived after 24 hours, with 6.28% of the female mites and 1.00% of the deutonymphs still alive. In the R. officinalis extract environment, adult mites could survive for to 48 hours, whereas immature stages could only survive up to 24 hours. The present findings indicate that herbal extracts derived from E. fortunei, R. officinalis, and Derris elliptica possess significant therapeutic potential for the clinical management of O. cynotis infestations in cats.
Keywords: Otodectes cynotis, Alternative, Herbal extract, Derris elliptica, Eupatorium fortunei, Rosmarinus officinalis

Abstract
Maintaining the health of dairy cows and ensuring their high fertility are urgent tasks for veterinary gynecology. Among the leading problems are inflammatory and functional gynecological diseases, resulting in long-term infertility. The study aims to compare the hematological parameters of cows during the insemination period and at the time of pregnancy diagnosis and determine the prevalence of subclinical endometritis among Holstein-Friesian cows. The study utilized the methods of endoscopy, ultrasound, transrectal palpation, and an express test, which were performed on Holstein cows at commercial facilities in the Almaty Region (Kazakhstan) and the Department of Obstetrics, Surgery, and Reproductive Biotechnology. Hematological tests were carried out at the Kazakh-Japan Innovation Center. Ultrasounds showed an increased size of the uterine cavity and thickened uterine walls. Research was conducted on cows in three age groups: 2.5–4, 5–7, and over 7 years old. The average prevalence of subclinical endometritis amounted to 8.63%. Throughout treatment, the hematological parameters of diseased cows changed. The study suggests that early diagnosis, effective treatment, and prevention are crucial in maintaining reproductive health and high milk yields considering cattle breed and lifestyle. The study analyzed the indications for the use and effectiveness of drugs, including Ihglyukovit, Tylometrinum, ASD-2F, and Uteroton. Low-level laser therapy was examined in terms of its contribution to the effectiveness of therapy for the disease under study. The benefits of multivitamin supplements were reviewed.
Keywords: Holstein breed, Subclinical endometritis, Hematology, Treatment.

Abstract
This study investigated the effects of DCT-2022 garlic extract on growth performance, diarrhea prevention, and immune response of chickens to Avian Influenza (AI) vaccines. The experiment was completely randomized with 5 treatments and 6 replications, each treatment had 120 CP2 chickens. Treatment TC was the control treatment without supplementation; treatments T10%, T15%, and T20% were supplemented with DCT-2022 at doses of 10%, 15%, and 20%, respectively; treatment TA was supplemented with amoxicillin/colistin at a dose of 1g/4L. The results showed that supplementation of 15% DCT-2022 improved feed intake of chickens at 9-11 weeks of age and body weight gain of chickens at 6-8 weeks of age. Moreover, serum samples were collected to determine antibodies against the AI virus 14 days after each vaccination using the hemagglutination inhibition assay. The results showed that supplementation of 15% DCT-2022 enhanced the immune response of chickens to the AI vaccine, with protection rates at 14 days after the first and second vaccination of 50 and 100%, respectively, as well as the GMT higher than that of the control treatment TC. Additionally, supplementing 15% DCT-2022 in drinking water could reduce the density of E. coli in the feces of chickens. Overall, supplementation of 15% DCT-2022 had beneficial effects on growth performance, immune response, and prevention of E. coli-induced diarrhea in chickens.
Keywords: Chickens, Diarrhea, Garlic extract, Growth performance, Immune response.

Abstract
Influenza has been considered a global health threat given its unpredictable and high mutation rate, with severe morbidity and mortality. Oseltamivir has been considered to treat both seasonal and pandemic influenza. In this regard, we performed a bibliometric analysis from 1999 to 2025 through the Scopus database to identify the leading journals, authors, articles, countries, institutions, years, hot spots, trends, classical and emerging research. We employed the VOSviewer and Bibliometrix package to visualize and analyze included studies. Of the 2001 records, 1410 records were involved in the analysis. 1410 papers were published by 7093 authors in 592 sources, with 26.03% of the published studies being internationally co-authored. We found that research interests in oseltamivir have changed over the years, especially in times of outbreaks of influenza infection and the COVID-19 pandemic. The bibliometric analysis of oseltamivir research reveals distinct thematic clusters Key clusters include mortality and epidemiology, safety in pregnancy and children, influenza subtypes, COVID-19, and antiviral agents. Early research (2004–2010) emphasized outbreaks and pandemic influenza, while later years (2011–2016) shifted toward oseltamivir resistance and neuraminidase inhibitors. Notably, post-2020 trends highlight emerging topics such as baloxavir, SARS-CoV-2, and COVID-19, reflecting expanded research into newer antivirals and pandemic applications. The thematic map further identifies niche areas like antiviral resistance and H1N1 subtypes, suggesting evolving priorities in influenza treatment and broader antiviral efficacy. Future studies should give attention to the safety and efficacy of oseltamivir in the new mutational versions of the influenza virus, as well as SARS-CoV-2.
Keywords: Oseltamivir, Influenza, Bibliometric analysis, COVID-19.

Abstract
Diabetes mellitus is a globally prevalent condition associated with increased morbidity and mortality. Poorly managed diabetes is characterized by elevated tissue factor (TF), a key initiator of coagulation and vascular inflammatory signaling. Emerging evidence indicates that direct oral anticoagulants (DOACs), such as rivaroxaban (RIV) and dabigatran (DAB), exhibit anti-inflammatory and antifibrotic properties beyond their anticoagulant effects. This study aimed to investigate the potential protective effects of RIV and DAB against streptozotocin (STZ)-induced pancreatic ?-cell loss in mice. Sixty Balb/c male mice were divided into six groups: control, RIV, DAB, STZ, RIV+STZ, and DAB+STZ. Serum insulin, blood glucose, platelet count, and protein expression of PI3K, p-AKT, PAR-2, fibrinogen, and ADAM10 were assessed. Histological analysis was performed to evaluate ?-cell integrity, extracellular matrix (ECM) deposition, and islet morphology. Treatment with STZ significantly decreased serum insulin and platelet counts, hyperglycemia, increased ECM deposition, increased PAR-2/fibrinogen expression, and islet atrophy. Co-treatment with STZ and RIV or DAB significantly improved insulin secretion, normalized glucose and platelet levels, modulated PI3K/p-AKT expression, reduced ADAM10 expression, and alleviated islet fibrosis. Treating mice with RIV and DAB exhibited protective effects against STZ-induced pancreatic islet damage, preserving ?-cell function and architecture, likely through modulation of inflammatory and fibrotic pathways. These results support the therapeutic potential of DOACs in mitigating ?-cell loss in diabetic models.
Keywords: Type 1 Diabetes; ADAM10; PAR-2; Insulin; Rivaroxaban; Dabigatran.