Volume 13, No. 6, 2024 (In Progress)

Flow Cytometric Evaluation of Different Monoclonal Antibody Clones against Rabbit Leukocytes
Doriana Beqiraj, Brixhilda Qyra, Ilir Dova, Albana Munga, Gabriele Brecchia, Enkeleda Ozuni, Laura Menchetti, Pëllumb Zalla, Stella Agradi, Egon Andoni, Susanna Draghi, Xhelil Koleci, Giulio Curone, Gerald Muça, Marta Castrica, Ornela Marko and Majlind Sulçe
Int J Vet Sci, 2024, 13(6): 737-741.
Abstract
Abstract
Many techniques are described to correctly identify rabbit leukocytes. Both automatic and manual counts are used for this purpose of identifying different subsets of rabbit white blood cells. However, other techniques such as Flow Cytometry are used in veterinary medicine to identify different surface and intracellular antigens of cells in a short period of time and with high accuracy. Antibodies are needed when a flow cytometric analysis takes place and it is mandatory that these antibodies react with the cells of the species of interest. Antibodies should always be chosen carefully in order to have the best performance when their use is predicted, especially when taking into consideration their species specificity. However, antibodies reacting to a specific species can react also to other species. To prove this statement, antibodies have to be tested in order to define and confirm their reactivity to other species. In this study thirty-three peripheral blood samples from healthy rabbits were collected and tested for the following antibodies: CD21 (Clone: LT21), CD5 (Clone: YKIX322.3), CD4 (Clone: YKIX302.9), CD8 (Clone: YCATE55.9), CD11b (Clone: M1/70). All antibodies report different species reactivity from rabbits. Results showed that only CD11b reacts with rabbit myeloid cells showing satisfactory results. The clone used in this study showed higher performance on monocytes compared to granulocytes. However, further studies with a higher caseload are warranted to confirm the results.
Keywords: Flow Cytometry, Rabbits, Antibodies, Leukocytes.
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Anticancer Activity of the 28.4 kDa Protein from Pediococcus pentosaceus SR6 in MCF-7 Breast Cancer Cell Line
I Wayan Suardana, Hevi Wihadmadyatami and Dyah Ayu Widiasih
Int J Vet Sci, 2024, 13(6): 742-748.
Abstract
Abstract
A preliminary study of total protein from the lactic acid bacteria (LAB) strain Pediococcus pentosaceus SR6 was proven to have an anticancer role in T47D cells. On the other hand, studies on the specific protein of this strain regarding its anticancer effect have so far never been revealed. Based on these considerations, this research was conducted. Research activities began by cultivating of LAB Pediococcus pentosaceus SR6 isolates, analyzing protein fragments using SDS-PAGE, isolating and electroeluting specific proteins from SDS-PAGE results, preparing MCF-7 breast cancer cell cultures, cytotoxic tests using the MTT cell proliferation and cytotoxicity kit method, and analyzing cell morphology via observation using the DAPI and Acridine Orange/Propidium Iodide (AO/PI) tests. The test results were then analyzed statistically using the SPSS25 program and then displayed in the form of Tables or Figures. The research results showed the 28.4kDa protein from Pediococcus pentosaceus SR6 was known to have anticancer activity with an IC50 of 5.42ppm. Their anticancer activity is selective because it is less toxic to normal cells which require a higher toxic dose, namely 28.36ppm. The protein has an ability to stimulate MCF-7 cells’ apoptosis and necrosis that is proved from the double staining with Acridine Orange/Propidium Iodide (AO/PI) and DAPI stainings. In conclusion, The 28.4kDa protein from LAB isolate Pediococcus pentosaceus SR6 was proven to be used as an breast anticancer compound with its selective ability against normal cells.
Keywords: Apoptosis, Pediococcus pentosaceus SR6, MCF-7 cell line, Vero cells, IC50.
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Molecular Identification of Fungal Pathogens causing Feather Infection in Parrots Suffering from the Psittacine Beak and Feather Disease (PBFD) Virus
Mohamed I AbdAllah, Sara M Elnomrosy, Nahla Hussien AbouEl Ela, Engy Farahat, Doaa D Khalaf, Khadijah A Altammar and Rasha MH Sayed-ElAhl
Int J Vet Sci, 2024, 13(6): 749-756.
Abstract
Abstract
Accurate identification of infectious molds from clinical specimens is crucial, as evidenced by the rising incidence of invasive fungal diseases in birds and their growing frequency. Fungal DNA was found in parrot feather samples linked to Psittacine Beak and Feather Disease (PBFD) virus infection following the detection of culture isolates. Internal transcribed spacer regions (ITS) have been employed as targets for phylogenetic research as they typically exhibit sequence variation between species, with modest change within strains of the same species. Numerous sequence variations were found when comparing ITS region sequences from reference and clinical isolates of six Aspergillus species, one Rhizopus arrhizus, and two Penicillium species, The addition of 5.8S rRNA gene sequences had little effect on the species as a whole due to the presence of little diversity between species in this region. Amplification, sequencing, and comparison with non-reference strain sequences in GenBank were performed on strains and clinical isolates of aspergilli and other fungi. Aspergillus and other species amplified ITS region had a size range of 540 to 608 base pairs (bp). A bootstrap of 1000 replications of every fungal isolate was also displayed on the phylogenetic tree, along with varying percentage rates among the fungal isolates.
Keywords: Circovirus, PBFD, Aspergillus, Rhizopus, Penicillium, Sequencing and Phylogenetic Tree.
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Nutrient Digestibility and Physiological Parameters of Crossbreed Weaner Rabbits Provided Dehydrated Bovine Rumen Digesta
Udeh Fredrick U, Oyeagu Chika E, Ani Augustine O, Adetunji Adewole T and Lewu Francis B
Int J Vet Sci, 2024, 13(6): 757-765.
Abstract
Abstract
The effect of dehydrated bovine rumen digesta (DBRD) on crossbreed weaner rabbits were tested. A total of 48 four-weeks-old crossbreed (New Zealand × Chinchilla) weaner rabbits of average initial weight of 0.88±0.02 to 0.92±0.02kg were assigned randomly into four dietary treatment groups with three replicates of four rabbits each. Dietary treatments include: DBRD0, DBRD5, DBRD10, and DBRD15. Body weight and daily weight gain of crossbreed weaner rabbits that consumed DBRD15 were higher (P<0.05) compared to those in other groups. Rabbits on DBRD15 consumed less (P<0.05) diet than those provided DBRD5 and control (DBRD0) diet. Rabbits that consume DBRD15 had the lowest (P<0.05) feed gain ratio value than those fed in other groups. Rabbits fed DBRD15 recorded the highest (P<0.05) digestibility of crude protein (CP), crude fiber (CF), and dry matter (DM). The digestibility of crude fat increased (P<0.05) in crossbreed weaner rabbits fed DBRD10 and DBRD15. Rabbits fed DBRD10 and DBRD15 had the highest (P<0.05) hemoglobin and packed cell volume production. Red blood cell production was significantly higher (P<0.05) for crossbreed weaner rabbits that consumed DBRD15. Rabbits fed dietary DBRD10 and DBRD15 had the lowest (P<0.05) lymphocyte counts than those provided the control (DBRD0) diet. The carcass weight was higher (P<0.05) for rabbits fed DBRD15. There was an increased (P<0.05) length of the large intestine for rabbits fed DBRD10 and DBRD15. Liver, heart, kidney, spleen, and lungs weights were higher (P<0.05) for rabbits fed DBRD10 and DBRD15. We concluded that 15% graded levels of DBRD improved the growth performance, nutrient digestibility, and health status of crossbreed weaner rabbits. Keywords: Abattoir Waste, Body Weight, Carcass, Crude Fiber, Hematology, Visceral Organ.
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Surveillance for Genetic Markers of Adaptation of SARS-CoV-2 in Dogs and Cats
Ida BK Suardana, Bayu K Mahardika and Gusti N Mahardika
Int J Vet Sci, 2024, 13(6): 766-771.
Abstract
Abstract
Surveillance for genetic markers of adaptation of SARS-CoV-2 in dogs and cats could help to predict the risk of variant emergence, that resulted from virus adaptation to the new host. The Delta variant of SARS-CoV-2 collected from dog and cat is of high interest, since this variant caused global major cases and fatality during the pandemic. All complete and high coverage sequence data of Delta variant of dog and cat origin, as well as samples of human origin SARS-CoV-1 were downloaded. All selected sequence data were subjected to Clustal Omega alignment. Evolutionary history was inferred by using the Maximum Likelihood method using Mega11. Species-specific markers indicating a probable species adaptation of the pandemic SARS-CoV-2 in dog and cat could not be identified. The markers found in many dog and cat samples is confirmed to be location specific markers that occurred in human origin virus.
Keywords: SARS-CoV-2; Companion animals; Adaptation; Genetic markers
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